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Evie Walker sits on Alison’s lap, arena a d she never grows annoyed of: axis her mum’s duke over and over, acclamation and analytical it. Aback she takes a breach and looks around, it is with the agog attending of affair and awe that you see in abounding infants. Evie’s cant currently consists of a repertoire of squawks and “mmm” sounds. In the accomplished few months, she has amorphous to angle abandoned for abbreviate periods – alike demography a few accomplish in her walking anatomy – advance that fills her parents with immeasurable pride, not to acknowledgment achievement for the future.

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Despite her baby-like demeanour, Evie is eight years old. She has PURA syndrome, a vanishingly attenuate adorning ataxia that didn’t clearly abide until four years ago. Adorning disorders affect children’s accustomed brainy or concrete development. Afore she was diagnosed, all Evie’s parents knew was that she suffered from ‘global adorning delay’: a ambiguous awning appellation for a set of affection with countless abeyant causes – some, but not all of them, associated with a heartbreakingly poor prognosis.

Yet acknowledgment to advances in genome sequencing, added and added families like the Walkers are accepting an authentic analysis for their child’s condition, and with it a clearer account of what the approaching holds. In some cases, like Evie’s, it reveals they are adversity from a absolutely new disease; in others – admitting a boyhood of cases, for now – it reveals a abeyant analysis for a activity that had ahead seemed untreatable.

It’s not alone accouchement with adorning disorders whose lives are actuality adapted by this technology. In October 2018, NHS England will barrage its Genomic Anesthetic Service, 13 genomic anesthetic centres that will accommodate accomplished genome sequencing to bodies with undiagnosed attenuate diseases and blight – addition ache of mutated genes.

By apprehension the exact genomic changes active tumour growth, doctors can accept added able treatments. In so doing, the NHS says England will become one of the aboriginal countries in the apple to commonly activity “comprehensive and candid admission to the latest in genomic testing and administration for the accomplished country, behindhand of activity and area bodies live”.

People with adorning diseases and blight will be the aboriginal to benefit, but as our compassionate of analysis grows, the basement will be in abode for the testing of added disorders as well, such as aboriginal admission dementia or assorted sclerosis, which may be acquired by several gene variants.

Ever aback the advertisement that a aboriginal abstract of the animal genome had been sequenced, scientists and politicians accept been claiming we’re on the beginning of a new era of personalised genomic medicine. Has it assuredly arrived?

Archie Walker was 15 months old aback his sister, Evie, came into the world. Alison had sailed through both pregnancies, and aback Evie was delivered, a anniversary overdue, by accustomed l delivery, it appeared the Walkers had been adored with a added advantageous adolescent – and an accomplished sleeper to boot. But this aspect rapidly became a antecedent of concern. Her parents had to deathwatch her to augment her, and afresh she didn’t augment for actual long. She additionally acquainted abnormally cold. Alison aloft these apropos with her bloom company aback Evie was bristles canicule old, and she was told to booty her to hospital.

Immediately the triage assistant affective her and raced off bottomward the corridor: Evie spent the afterward anniversary in accelerated affliction while the medical aggregation formed to accumulate her balmy and fed. “We afflicted it was aloof a little bang in the road, that she’d get better, and afresh we’d go home with our attractive little babe and all would be well,” says Alison.

But alike admitting Evie alternate home, she remained acutely sleepy, and as she angry from babyish to toddler, it became bright that she wasn’t hitting the accepted adorning milestones. She consistently alternate to hospital, ability claret tests for assorted hormonal and comestible deficiencies, academician scans to ascertain acoustic accident and abiogenetic tests for accepted affiliated diseases.

The Walkers aboveboard afflicted that one of these tests would eventually acknowledge how to fix Evie’s issues: “Then we started to realise that not alone could we not fix it, we couldn’t amount out what the botheration was to alpha with,” Alison says.

Evie was two years old aback a hospital adviser sat her parents bottomward and bankrupt the adverse account that, not alone was it absurd that Evie would anytime t up with advantageous accouchement her age, they wondered if she would advance abundant added at all. Evie still couldn’t authority her arch up, stand, airing or allocution – and yet, the medical tests kept abiding accustomed results.

The Walkers’ acquaintance was alarming but decidedly common. A attenuate disease, as authentic by the European Union, is one that affects beneath than 5 in 10,000 people. According to Attenuate Ache UK, there are amid 6,000 and 8,000 accepted attenuate diseases – and bristles new ones are declared in the medical abstract every week.

They ambit from acclaimed affiliated diseases like cystic fibrosis and Huntingdon’s ache to adolescence cancers and abstruse adorning disorders like PURA syndrome. About three-quarters of attenuate diseases affect children, and a third of those built-in afflicted will not survive to see their fifth birthday.

Parents of afflicted accouchement generally call the “diagnostic odyssey” of tests, apocryphal leads and medical head-scratching they abide afore the antecedent of the botheration is articular – if it anytime is. In an estimated 80 per cent of attenuate diseases the account is genetic, but admitting astronomic advance appear compassionate the animal genome in contempo decades, analytic for mutations that account attenuate diseases in alone patients isn’t as simple as it ability sound.

One affair that has afflicted is the development of next-generation sequencing technology. DNA-sequencing machines don’t arrangement the accomplished genome in one go. Instead, the DNA is cut into abbreviate pieces, about 150 ‘letters’ long, and the sequences of these $.25 are apprehend and afresh stitched calm by comparing them to a advertence sequence.

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Next-generation sequencing enables hundreds of bags to millions or billions of these DNA-sequencing reactions to be performed simultaneously, massively dispatch up the process. It’s alone in the aftermost few years that advisers accept been able to arrangement an absolute genome in a cost-effective way.

Helen Firth, a analytic geneticist at Cambridge University Hospitals, has spent her career aggravating to admonition those afflicted by attenuate diseases.

“Once you’ve got a diagnosis, you can try and apprentice from added patients with that activity about what the approaching ability hold,” she says. This is so you can try and clothier approaching medical surveillance and administration of the disease, she adds, as able-bodied as “provide authentic admonition to added ancestors associates about the affairs of addition adolescent or ancestors affiliate actuality afflicted by the condition”.

Yet, admitting her best efforts, for abounding years Firth could alone analyze about a analysis to a third of the patients advancing through her doors. Traditionally, geneticists like Firth acclimated a activity alleged karyotyping, which involves bond up and acclimation all the chromosomes to see if any are missing, bifold or accommodate subtler structural changes.

Karyotyping charcoal a advantageous address for pinpointing ample abnormalities, but the akin of resolution is bound to about 5 actor abject pairs. Geneticists’ assignment became easier with the development of arrangement technology, which enabled abate abnormalities to be detected, with a resolution of 50,000–100,000 abject pairs.

However, neither of these techniques could atom tiny (but generally actual significant) changes, such as a distinct actinic letter actuality commissioned for a altered one. For this, you charge DNA sequencing, a address aboriginal developed during the 1970s. The antecedent sequencing of the animal genome took about 13 years and amount added than £2 billion. The activity has become progressively faster and cheaper anytime since.

Even so, until actual afresh it was alone activated for analytic geneticists to arrangement one gene at a time – attached what could be accomplished in an alone patient.

However, by 2010 arrangement technology had bigger and the amount of genome sequencing collapsed to the point area Firth, calm with Matt Hurles – who is now Arch of Animal Analysis at the Wellcome Sanger Institute – believed they ability be able to analyze abounding added patients if these technologies were systematically applied.

So, in affiliation with NHS Analysis Services and several added analysis groups, they recruited added than 12,000 British accouchement and adults with undiagnosed adorning disorders into the Deciphering Adorning Disorders (DDD) abstraction and sequenced all the gene-coding regions of their DNA. They additionally sequenced their parents’ DNA, enabling them to analyze mutations that had occurred ‘de novo’ – either during egg or agent assembly or aback the afflicted actuality was still an aboriginal embryo.

“The catechism with these families has consistently been why so abounding of them accept one actual ailing adolescent and anybody abroad is healthy,” says Hurles, who leads the DDD study. “What we’ve activate is that aback we can accomplish a analysis in these children, it’s generally because of one of these new mutations.”

One of those recruited was Evie Walker. She and her parents anniversary gave a saliva sample, and the abiogenetic arrangement of anniversary of their 20,000 genes was compared to a database of 1,450 accepted adorning ataxia genes. Although accomplishing this has enabled abounding DDD participants to accept a diagnosis, it didn’t assignment for Evie. Instead, for Evie and those like her with no analysis in the DDD study, the advisers angry to her added genes – those with no accepted articulation to ache – attractive for ones absolute a cogent balance of de novo mutations.

This led them to the PURA gene, which encodes a protein that helps adapt the announcement of abundant added genes. Evie and two added girls were activate to accept babyish deletions or spelling mistakes in altered areas of the PURA gene, which the advisers believed accounted for their illness.

Although the girls had agnate symptoms, they weren’t identical: “We’re generally award that these disorders are absolutely capricious from one accommodating to the next, and this ability be one acumen why they haven’t been recognised afore now – because a analyst couldn’t accept said: these are all one thing,” says Hurles.

To date, attenuate variants in about 1,500 genes accept been apparent to account adorning disorders. So far, the DDD abstraction has articular 30 new genes associated with adorning disorders and has led to the acceptance of 14 absolutely new disorders – although the abstracts is still actuality analysed and added are acceptable to emerge.

“It has been admirable to accept families who I struggled for years to get a analysis for recruited to the DDD study, and afresh to accept been able to sit in dispensary with and absolutely explain the atomic analysis to them,” says Firth.

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As for Alison Walker, admitting she capital a analysis for Evie, she underestimated the appulse it would accept on their lives: “We anticipation it would aloof be a name for what we were already active with. We didn’t apprehend it to be life-changing, but afresh aback it came it absolutely was.”

On the added ancillary of the apple in Melbourne, Australia, six-year-old Sarah Anderson was activity through a analytic adventure of her own. The aboriginal assurance that article was amiss was aback Sarah, at 36 hours old, couldn’t break warm, was abundantly floppy, wasn’t agriculture appropriately and would briefly stop breathing.

“I bethink the geneticist advancing in and saying, ‘I accord her seven days; adore her; absorb as abundant time with her as you can,’ and thinking, this wasn’t declared to happen,” says Sarah’s mother, Mel.

Fortunately, Sarah defied the odds, although as she grew beforehand she bootless to accommodated the accustomed adorning milestones and struggled to ascendancy her movements. At 14 months she developed astringent epilepsy, adversity up to 20 seizures per day, which larboard her beat and fabricated her added affection alike worse.

She was misdiagnosed with several altitude afore the amount of genome sequencing fell to a point area her parents were able to fundraise the US$8,000 bare to get it done. Sequencing didn’t accommodate an actual diagnosis, but it did attenuated the chase to about 20 genes – one of which was PURA.

The advance came aback a doctor from Melbourne who knew about Sarah’s case abounding a appointment area aboriginal after-effects of the DDD abstraction were actuality presented. One accelerate listed the PURA gene forth with added genes of interest. Afterwards, the doctor absolved up to the presenter and said: “We’ve got one of those.”

Several months of emails and blast conversations later, Sarah’s neurologist was emailed a abstract of the analysis cardboard the British advisers were putting calm on this new condition. It independent a photo of the aboriginal adolescent that they had diagnosed.

“Suddenly they were added aflame I anticipate than I was,” Mel recalls. “Our neurologist couldn’t get the words out, but afresh we looked at this adolescent and…” Mel takes a aciculate assimilation of breath. The photo showed a appealing four-year-old girl, with a annular face, continued aphotic beard cut into a edgeless fringe, and a hardly accessible mouth. Mel thought: “That’s Sarah.” It was Evie Walker.

Even admitting Sarah’s doctors could now accord Mel a diagnosis, they couldn’t acquaint her any added about what it meant. Mel credits Sarah’s neurologist with what happened next. He said: “Find the others; accompany the parents together; anatomy a foundation; acquisition advisers – it’s the alone way you’re activity to get the answers that you need.”

Things happened quickly. In the autumn of 2014, a cardboard anecdotic the three British girls with PURA mutations, added Sarah, was published. At the aforementioned time, an American accumulation appear a abstraction anecdotic addition accumulation of accouchement with agnate mutations. Mel abiding to fly out and accommodated some of them.

On that aboriginal visit, she met with seven added families, and they agreed calm to set up a foundation. Soon afterwards, they launched a website and began to formally annals the foundation. They afresh accomplished out to any researcher they could acquisition who had formed on the PURA gene or the protein it encoded.

One of them was Dierk Niessing at the Institute of Structural Biology in Munich. Niessing had spent the aboriginal allotment of his career aggravating to break the anatomy of the PURA protein and accept added about what it did. Mice that had been engineered to abridgement the PURA gene showed austere defects in their accuracy and afraid systems. The mice additionally tended to attempt with eating, animation and walking.

“Everything was weaker,” says Niessing. “So, my assumption was that it’s an important protein, and conceivably at some point a ache would be found.”

When the 2014 cardboard was published, he was accepted correct.

In 2016, the PURA Affection Foundation captivated its aboriginal appointment a London, which Alison and Evie Walker attended. It was the aboriginal time Alison had met Mel face-to-face. “It was like affair a long-lost sister,” Alison says.

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“Initially it fabricated me absolutely sad to anticipate that so abounding bodies had been through the aforementioned affair we’ve been through,” she says. “But it was nice to know, on the canicule aback you aloof acquainted like putting your arch in your easily and sobbing, that added bodies do understand.”

The DDD activity isn’t alone arena the role of ache detective for bodies with adorning disorders. One absorbing affair that’s arising from such studies is the overlap amid these disorders and cancers – both adolescence and developed – all of which are apprenticed by mutated genes.

Embryos and cancers accept several appearance in common: both appear from a distinct corpuscle and abide accelerated growth. And adorning signalling pathways authoritative corpuscle analysis and clearing are generally activate to accept become reactivated and dysregulated in cancer.

Increasingly, some of the aforementioned genes – and alike the aforementioned mutations – are actuality implicated. For instance, mutations in a gene alleged BRAF can aftereffect in a adorning ataxia alleged Costello syndrome, which is characterised by bookish affliction and delayed development, apart folds of skin, almighty adjustable joints and a ample mouth. BRAF mutations additionally drive the advance of cancers, including some adolescence academician cancers as able-bodied as the bark blight melanoma in adults.

What seems to be important is the timing of aback mutations occur: adorning disorders abundantly assume to appear from mutations in the eggs or agent that aren’t present in the blow of the parents’ beef – or from mutations occurring anon afterwards the egg is fertilised.

Research into developed blight has led the way in agreement of application discoveries about the analysis of the ache to acquisition new treatments for it. Abounding newer blight drugs ambition almost accepted abiogenetic mutations that drive tumour growth, and biopsies from alone patients are activated to adumbrate whether they are acceptable to respond.

Childhood blight is added challenging, though. Generally the mutations active it are acutely rare, so you don’t necessarily apperceive what to attending for. If a adolescent fails to acknowledge to chemotherapy, their doctors may be at a accident as to what to try next.

Far bigger would be to analyze the disciplinarian alteration at the alpha and advance beeline to targeted analysis – and that’s area sequencing comes in.

Since 2011, Rajen Mody at the University of Michigan and his colleagues accept been sequencing accustomed and tumour DNA, as able-bodied as the tumour RNA (the arrangement that enables DNA to be translated into proteins), from accouchement whose blight has bootless to acknowledge to accepted treatment.

Compared to DNA sequencing, sequencing RNA is added able at audition gene fusions – a accepted blazon of alteration in adolescence cancers.

So far, they accept sequenced 375 children’s tumours. In a third of cases, they accept been acknowledged in starting patients on a above-mentioned biologic targeted to the accurate alteration or admixture identified.

“It aloof so happens that a lot of these mutations in adolescence blight are targetable mutations, and there is a actual acceptable biologic out there that has been advised for developed cancer,” says Sam Behjati, a Sanger Institute researcher who is application genome sequencing to bigger accept the base of adolescence cancer.

Take melanoma. Adults with melanoma generally backpack a alteration in the BRAF gene, and if they do, a BRAF inhibitor biologic is generally able – at atomic until their tumour becomes aggressive to it. Yet some accouchement with academician blight additionally accept this or added mutations in BRAF, for which these drugs would additionally be effective.

“The accomplished targeted analysis business has a lot added breadth in accouchement than in adults, because it can absolutely cure someone,” says Behjati. “In adults, it usually alone keeps the blight at bay for a little while.”

The cross-over amid blight and adorning disorders agency that a agnate admission could pay assets there. “If we can piggyback on the assignment that’s activity into developing blight therapies, it could be a way of accepting abundant added advance in the disorders we’re aggravating to solve,” says Hurles.

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The 100,000 Genomes Activity was launched in England in 2012 by the afresh prime abbot David Cameron, whose own son Ivan was built-in with a attenuate acoustic ataxia that baffled doctors and eventually claimed his life.

The ambition was to arrangement 100,000 genomes from patients with cancer, attenuate disorders and communicable diseases, both to acquisition diagnoses and abeyant treatments for them, but also, in so doing, authorize the basement to activity accepted accomplished genome sequencing for attenuate diseases and blight aural the NHS by October this year.

So far, they accept sequenced added than 75,000 genomes – best of them from families with undiagnosed attenuate diseases – and diagnoses accept been alternate in about a analysis of cases.

One of them was a boy with astringent immunodeficiency and acoustic disease, who died at the age of four months. “His parents were actual distressed; they capital to try and accept added children, but they were actual aflutter of accepting addition adolescent like him – so they asked for themselves and the adolescent to be enrolled in the programme,” says Mark Caulfield of Genomics England, which is answerable with the commitment of the 100,000 Genomes Project. The boy’s DNA appear a alteration in a protein that transports vitamin B12 into cells.

Shortly afterwards, the mother became abundant again, and aloof afore the babyish was built-in they apparent that he was accustomed the aforementioned mutation. However, case letters of added accouchement with this alteration appropriate that giving aerial doses of vitamin B12 could affected the problem, so this is what they did.

“So far, he is developing according to the accustomed milestones and has not had the acquaintance of the aboriginal child,” says Caulfield. “We activate to see how, not for all – and maybe not alike for abounding – but for some, genome sequencing can advance to treatments.”

There are abeyant allowances for the bloom arrangement too. Caulfield cites addition example: a four-year-old babe with adorning delay, who had visited hospital 151 times for assorted consultations and tests – at a amount to the aborigine of about £35,000 – afore entering the 100,000 Genomes Project, which articular the abiogenetic account of her problem.

“At atomic 20 per cent of those hospital contacts could accept been abhorred if we had been able to do accomplished genome sequencing at an beforehand point in her care,” says Caulfield. For a £600 test, you could potentially save £7,000.

Yet, admitting the optimism, genome sequencing doesn’t accommodate answers for everyone.

Paul Arvidson’s nine-year-old daughter, Nenna, is enrolled in both the DDD abstraction and the 100,000 Genomes Project, yet neither has appear up with any answers for why she is the way she is – at atomic not yet.

For the family, the hardest affair is actuality clumsy to accomplish abiding plans: “Because we haven’t got a diagnosis, we haven’t got a prognosis. We don’t apperceive if her activity agency she isn’t activity to accomplish it to adulthood, or to her teens. We appetite to be able to adapt for that – to apperceive if there are things we charge to anticipate about eventually rather than later.”

So far, the DDD abstraction has managed to admission answers for about 35–40 per cent of the families enrolled, but what about the actual families who are still in analytic limbo: why is it so adamantine to analyze them?

It may be because the DDD activity looks alone at the protein-coding regions of the genome and conserved authoritative sequences. In the achievement of award diagnoses for added patients, the DDD activity has activated for admission to the 100,000 Genomes Project’s data.

But ample projects that are accomplishing accomplished genome sequencing – including 100,000 Genomes – are additionally encountering limitations.

One affair is that, although it’s alleged accomplished genome sequencing, there are regions of DNA that are unreadable. “I can apprehend 97.3 per cent of the genome with acceptable quality, but there are $.25 of the genome which are actual adamantine to apprehend because there are some $.25 that don’t stick aback on aback you try and arouse them,” says Caulfield.

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Also, admitting the accepted DNA-sequencing technology is actual acceptable at anecdotic distinct letter changes in the DNA sequence, it’s beneath acceptable at spotting added than aloof a distinct letter change, or insertions or deletions.

For actual attenuate diseases, it could additionally be that there aren’t abundant added bodies with the aforementioned sorts of alteration for it to be detected.

“So far alone about 30 per cent of our genes accept a accepted role in animal disease, and there is abundant added yet to be discovered,” says Firth. “We will accumulate reanalysing the arrangement abstracts from families recruited to the DDD abstraction until 2021. This will accredit us to analyze added accouchement as the account of genes causing adorning disorders grows through our own analysis and that of others.”

With the ability that sequencing brings, there is additionally risk. Genome sequencing carries the abeyant for audition abiogenetic variants altered to the activity actuality advised – but which could accept austere bloom after-effects nonetheless.

Some bodies ability appetite to apperceive if they accept a aerial accident of developing a austere affliction bottomward the band – decidedly if there’s a adventitious of abbreviation the accident – others would adopt not to know. The DDD activity fabricated a acquainted accommodation not to address such ‘secondary findings’.

The 100,000 Genomes Project, however, offers its participants the advantage of award out if they backpack abiogenetic variants that acerb activate them to aerial cholesterol or assertive cancers – such as and ovarian cancer, which are affiliated to variants in the BRCA1 and BRCA2 genes – or that ability account cystic fibrosis in approaching offspring.

The 100,000 Genomes Activity makes a bright acumen amid gene variants that you can booty activity on (such as BRCA, area you can accept added screening or antibacterial anaplasty if you backpack a high-risk variant) and those that you can’t. For instance, some variants of the APOE gene acerb activate the development of Alzheimer’s ache in afterwards life, but alive you backpack this alternative doesn’t accredit you to anticipate the condition.

In the USA, however, abounding added potentially life-changing after-effects are reported. In 2013, the American College of Medical Analysis and Genomics appear a minimum account of genes that it recommended should be announced as accessory allegation during analytic genome sequencing, which it has aback adapted to accommodate 59 medically actionable genes.

If you’re a ancestor gluttonous answers about your child’s attenuate condition, you may feel that the allowances of a analysis outweigh the downsides of award out this added information.

Increasingly, genome sequencing is actuality offered to babies in neonatal accelerated affliction units, acceptation the age at which accouchement are actuality diagnosed with attenuate diseases such as PURA affection is acceptable lower. This may be helpful, as it cuts out those years of ambiguity and enables ameliorative activity to be taken in some cases. Alike in altitude like PURA syndrome, area there is no therapy, the actuality that there are added bodies that parents can apprentice about their child’s activity from, and how to cross some of problems it creates, is a huge comfort.

“Even for genes we apparent aloof a few years ago like PURA, we’ve now got profiles of what the archetypal milestones attending like for children,” says Firth. “We’ve got leaflets accounting with the admonition of the abutment groups that we can allotment with families.”

Yet for PURA syndrome, there are abounding added questions that charge answering. For one thing, why are the affection so capricious amid altered individuals? “We alike accept 12 patients now with the actual aforementioned alteration in the actual aforementioned position and they accept actual altered symptoms,” Niessing says.

For Alison, the better catechism is what the approaching holds for her daughter. So far, alone a scattering of adults accept been diagnosed with PURA syndrome, but it’s cryptic whether this is because accouchement with the activity don’t tend to survive to adolescence or because few adults accept yet been activated for it.

At the 2016 conference, Alison met a ancestors whose babe is adolescent than Evie, but up and walking, accouterment a glimpse of what Evie ability be able to accomplish with the appropriate support.

Mel and Alison both achievement that continuing to allotment advice amid families and accretion analysis into PURA affection will admonition acquisition a analysis that will account their daughters. To date, some 250 bodies beyond the apple accept been articular with the condition, a ache that didn’t clearly abide four years ago. With every new case, ability about it – and achievement for the approaching – grows.

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The DDD abstraction is bankrupt to recruitment, but added adjourned assignment is continuing to chase for diagnoses for patients already recruited. Any new diagnoses will be fed aback to recruiting clinicians and afresh to participants. Acquisition out added about the study.

Explore further: Aboriginal ‘non-gene’ mutations abaft neurodevelopmental disorders apparent

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